• Product name
  • Description
    Rabbit polyclonal to NEK9
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human NEK9 conjugated to KLH

  • Positive control
    • 293 cell lysate, mouse heart tissue lysate, human hepatocellular carcinoma tissue.



Our Abpromise guarantee covers the use of ab71812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).


  • Function
    Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
  • Tissue specificity
    Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.
  • Sequence similarities
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
    Contains 6 RCC1 repeats.
  • Developmental stage
    Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
  • Domain
    Dimerizes through its coiled-coil domain.
  • Post-translational
    Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1995 antibody
    • APUG antibody
    • DKFZp434D0935 antibody
    • LCCS10 antibody
    • MGC138306 antibody
    • MGC16714 antibody
    • NC antibody
    • NEK 9 antibody
    • Nek8 antibody
    • nek9 antibody
    • NEK9_HUMAN antibody
    • Nercc 1 kinase antibody
    • NERCC antibody
    • NERCC1 antibody
    • Nercc1 kinase antibody
    • Never in mitosis A related kinase 9 antibody
    • Never in mitosis A-related kinase 9 antibody
    • NIMA (never in mitosis gene a) related kinase 9 antibody
    • NIMA related kinase 8 antibody
    • NIMA related kinase 9 antibody
    • NIMA related kinase Nek8 antibody
    • NimA related protein kinase 9 antibody
    • NIMA-related kinase 8 antibody
    • NimA-related protein kinase 9 antibody
    • Serine/threonine protein kinase Nek9 antibody
    • Serine/threonine-protein kinase Nek9 antibody
    see all

Anti-NEK9 antibody images

  • All lanes : Anti-NEK9 antibody (ab71812) at 1/100 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : mouse heart tissue lysate

    Lysates/proteins at 12.5 µg per lane.

    Predicted band size : 107 kDa
    Observed band size : 107 kDa
  • ab71812 at 1/50 dilution, staining NEK9 in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

References for Anti-NEK9 antibody (ab71812)

This product has been referenced in:

See 1 Publication for this product

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