Overview

  • Product nameAnti-NEK9 antibody
    See all NEK9 primary antibodies
  • Description
    Rabbit polyclonal to NEK9
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: GGGGGEEEDS QQESETPDPS GGFRGTMEAD RGMEGLISPT EAMGNSNGAS , corresponding to internal sequence amino acids 756-805 of Human NEK9 (NP_149107)

  • Positive control
    • THP-1 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab89853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 107 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
  • Tissue specificityMost abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.
  • Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
    Contains 6 RCC1 repeats.
  • Developmental stageExpression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
  • DomainDimerizes through its coiled-coil domain.
  • Post-translational
    modifications
    Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1995 antibody
    • APUG antibody
    • DKFZp434D0935 antibody
    • LCCS10 antibody
    • MGC138306 antibody
    • MGC16714 antibody
    • NC antibody
    • NEK 9 antibody
    • Nek8 antibody
    • nek9 antibody
    • NEK9_HUMAN antibody
    • Nercc 1 kinase antibody
    • NERCC antibody
    • NERCC1 antibody
    • Nercc1 kinase antibody
    • Never in mitosis A related kinase 9 antibody
    • Never in mitosis A-related kinase 9 antibody
    • NIMA (never in mitosis gene a) related kinase 9 antibody
    • NIMA related kinase 8 antibody
    • NIMA related kinase 9 antibody
    • NIMA related kinase Nek8 antibody
    • NimA related protein kinase 9 antibody
    • NIMA-related kinase 8 antibody
    • NimA-related protein kinase 9 antibody
    • Serine/threonine protein kinase Nek9 antibody
    • Serine/threonine-protein kinase Nek9 antibody
    see all

Anti-NEK9 antibody images

  • Anti-NEK9 antibody (ab89853) at 1 µg/ml + THP-1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 107 kDa

References for Anti-NEK9 antibody (ab89853)

ab89853 has not yet been referenced specifically in any publications.

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