The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionCouples NMDA receptor signaling to the nucleus. Influences outgrowth of olfactory axons and migration of LHRH neurons.
Tissue specificityHighly expressed in adult and fetal brain, testis and kidney.
Involvement in diseaseDefects in NELF may be associated with idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
Cellular localizationCell membrane. Nucleus. Found on the outside of the LHRH cell membrane and axons projecting from the olfactory pit and epithelium. Myristoylation is a prerequisite for extranuclear localization.