Overview

  • Product nameAnti-NELFe antibody
    See all NELFe primary antibodies
  • Description
    Sheep polyclonal to NELFe
  • Tested applicationsSuitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab28038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/800.
IP Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionEssential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
  • Tissue specificityWidely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the RRM NELF-E family.
    Contains 1 RRM (RNA recognition motif) domain.
  • DomainThe RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Major histocompatibility complex gene RD antibody
    • Negative elongation factor E antibody
    • Negative elongation factor polypeptide E antibody
    • NELF e antibody
    • NELF-E antibody
    • Nelfe antibody
    • NELFE_HUMAN antibody
    • Nuclear protein antibody
    • RD antibody
    • RD protein antibody
    • RD RNA binding protein antibody
    • RDP antibody
    • RNA-binding protein RD antibody
    see all

Anti-NELFe antibody images

  • All lanes : Anti-NELFe antibody (ab28038) at 1/1000 dilution

    Lane 1 : NELFe-GST fusion protein
    Lane 2 : HeLa cell nuclear extract

    Secondary
    donkey anti-sheep peroxidase linked at 1/20000 dilution

    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • HeLa cells stained with: 1. HCM antibody ab28038 diluted 1/800 and donkey anti-sheep Alexa 647 secondary antibody diluted 1/1000 2. DAPI

References for Anti-NELFe antibody (ab28038)

ab28038 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"