Anti-NELFe antibody (ab84986)

Overview

• Product name
  Anti-NELFe antibody
  See all NELFe primary antibodies
• Description
  Rabbit polyclonal to NELFe
• Tested applications
  Suitable for: IHC-Pmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
• Immunogen

  Synthetic peptide, corresponding to a region between residue 330 and 380 of human NELFe (NP_002895.3)

• Positive control
  • Human Breast Carcinoma, human Placenta

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  Preservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Elongation factors

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
• Tissue specificity
  Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
• Sequence similarities
  Belongs to the RRM NELF-E family.
  Contains 1 RRM (RNA recognition motif) domain.
• Domain
  The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
• Post-translational
  modifications
  Sumoylated.
• Cellular localization
  Nucleus.

• Target information above from: UniProt accession P18615 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 7936 Human
  • Omim: 154040 Human
  • SwissProt: P18615 Human
  • Unigene: 423935 Human
• Alternative names
  • Major histocompatibility complex gene RD antibody
  • Negative elongation factor E antibody
  • Negative elongation factor polypeptide E antibody

  • NELF e antibody
  • NELF-E antibody
  • Nelfe antibody
  • NELFE_HUMAN antibody
  • Nuclear protein antibody
  • RD antibody
  • RD protein antibody
  • RD RNA binding protein antibody
  • RDP antibody
  • RNA-binding protein RD antibody

  see all

Protocols

• Immunohistochemistry protocols