Overview

  • Product name
  • Description
    Rabbit polyclonal to NELL2
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human NELL2.

    (Peptide available as ab93303.)

  • Positive control
    • This antibody gave a positive signal in mouse cerebellum and hippocampus tissue lysates, and in the following whole cell lysates: U87mg; SHSY5Y; SKNBE. It also gave a positive signal in FFPE human cerebral cortex tissue sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).

Target

Images

  • All lanes : Anti-NELL2 antibody (ab80885) at 1 µg/ml

    Lane 1 : Mouse Hippocampus Tissue Lysate
    Lane 2 : Mouse Cerebellum Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 91 kDa
    Observed band size : 91 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab80885 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • IHC image of NELL2 staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80885, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-NELL2 antibody (ab80885) at 1 µg/ml

    Lane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 91 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,80 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutesHuman Protein kinase C-binding protein NELL2 precursor (NELL2) has a predicted molecular weight of 91 kDa, however it is extensively glycosylated (SwissProt data) which may affect the migration of the protein, accounting for the banding around the 100 kDa region. A lower band is observed at 80 kDa, which could correspond to a cytosolic form of NELL2 (cNELL2) that is generated by alternative splicing (Hwang et al, 2007). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

This product has been referenced in:
  • Araki A  et al. Chromosome 1q gain and tenascin-C expression are candidate markers to define different risk groups in pediatric posterior fossa ependymoma. Acta Neuropathol Commun 4:88 (2016). IHC . Read more (PubMed: 27550150) »
  • Zakrzewska M  et al. Altered MicroRNA Expression Is Associated with Tumor Grade, Molecular Background and Outcome in Childhood Infratentorial Ependymoma. PLoS One 11:e0158464 (2016). IHC-P ; Human . Read more (PubMed: 27390862) »

See all 2 Publications for this product

Customer reviews and Q&As

thank you for your enquiry. I can confirm that this peptide is provided on specific request, we do not hold stock of this peptide at any time. Therefore, it will take 4 - 6 weeks for it to arrive with the customer after an order is placed as st...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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