The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 19 kDa).
Use a concentration of 20 µg/ml.
FunctionDisplays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme.
Sequence similaritiesBelongs to the cytochrome b5 family. MAPR subfamily. Contains 1 cytochrome b5 heme-binding domain.
DomainThe cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.
ab74474 at 10 µg/ml staining NENF in rat kidney tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
References for Anti-NENF antibody (ab74474)
This product has been referenced in:
Stefanska B et al. Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets. Clin Cancer Res20:3118-32 (2014).
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