Anti-Neomycin Phosphotransferase 2 antibody [4B4D1] (ab60018)

Overview

  • Product name
    Anti-Neomycin Phosphotransferase 2 antibody [4B4D1]
    See all Neomycin Phosphotransferase 2 primary antibodies
  • Description
    Mouse monoclonal [4B4D1] to Neomycin Phosphotransferase 2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Escherichia coli
  • Immunogen

    Recombinant full length protein

Properties

Applications

Our Abpromise guarantee covers the use of ab60018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
ELISA 1/10000.

Target

  • Relevance
    Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
  • Database links
    • Alternative names
      • Aminoglycoside 3' phosphotransferase antibody
      • APH(3') II antibody
      • APH(3')II antibody
      • aphA antibody
      • EC 2.7.1.95 antibody
      • Kan antibody
      • Kanamycin kinase type II antibody
      • Kanamycin kinase, type VIII antibody
      • kanamycin resistance gene antibody
      • Neo antibody
      • Neomycin kanamycin phosphotransferase type II antibody
      • Neomycin kanamycin phosphotransferase type VIII antibody
      • Neomycin phosphotransferase II antibody
      • Neomycine phosphotransferase antibody
      • NPT II antibody
      • NPTII antibody
      see all

    Anti-Neomycin Phosphotransferase 2 antibody [4B4D1] images

    • Anti-Neomycin Phosphotransferase 2 antibody [4B4D1] (ab60018) at 1/500 dilution + Recombinant Neomycin Phosphotransferase 2 expressed in E. coli.

      Predicted band size : 29 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    References for Anti-Neomycin Phosphotransferase 2 antibody [4B4D1] (ab60018)

    This product has been referenced in:
    • Bentham MJ  et al. NS2 is dispensable for efficient assembly of hepatitis C virus-like particles in a bipartite trans-encapsidation system. J Gen Virol 95:2427-41 (2014). Read more (PubMed: 25024280) »
    • Holler CJ  et al. Bridging integrator 1 (BIN1) protein expression increases in the Alzheimer's disease brain and correlates with neurofibrillary tangle pathology. J Alzheimers Dis 42:1221-7 (2014). Read more (PubMed: 25024306) »

    See all 9 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Arabidopsis thaliana Tissue lysate - whole (seedlings)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    100 µg
    Treatment
    protease inhibitor
    Specification
    seedlings
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Nico Dissmeyer

    Verified customer

    Submitted Aug 03 2015

    I am very pleased to hear you would like to accept our offer and test ab60018 in ICC-IF

    DISCOUNT CODE: #####
    Expiration date: ######

    This code will give you: 1 free primary antibody before the expiration date. To redeem this ...

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    Thank you for your enquiry.

    I can confirm that to our knowledge, ab33595 and ab60018 have not yet been tested in ICC (immunocytochemistry staining of cell culture), IHC-Frozen sections (IHC-Fr) or IHC-Paraffin tissue sections (IHC-P).
    ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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