Anti-kan antibody [4B4D1] (ab60018)
Key features and details
- Mouse monoclonal [4B4D1] to kan
- Suitable for: ELISA, WB
- Reacts with: Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-kan antibody [4B4D1] -
Description
Mouse monoclonal [4B4D1] to kan -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Escherichia coli -
Immunogen
Recombinant full length protein corresponding to kan.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2 -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4B4D1 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa). |
Target
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Relevance
Neomycin Phosphotransferase 2 (kan) confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids. -
Database links
- SwissProt: P14509 Escherichia coli
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Alternative names
- Aminoglycoside 3' phosphotransferase antibody
- APH(3') II antibody
- APH(3')II antibody
see all
Images
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Anti-kan antibody [4B4D1] (ab60018) at 1/500 dilution + Recombinant kan expressed in E. coli.
Predicted band size: 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (11)
ab60018 has been referenced in 11 publications.
- Li X et al. Dehydrin MtCAS31 promotes autophagic degradation under drought stress. Autophagy N/A:1-16 (2019). PubMed: 31362589
- Kallehauge TB et al. Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion. Sci Rep 7:40388 (2017). PubMed: 28091612
- Bentham MJ et al. NS2 is dispensable for efficient assembly of hepatitis C virus-like particles in a bipartite trans-encapsidation system. J Gen Virol 95:2427-41 (2014). PubMed: 25024280
- Holler CJ et al. Bridging integrator 1 (BIN1) protein expression increases in the Alzheimer's disease brain and correlates with neurofibrillary tangle pathology. J Alzheimers Dis 42:1221-7 (2014). PubMed: 25024306
- Wong TT et al. Production of zebrafish offspring from cultured female germline stem cells. PLoS One 8:e62660 (2013). IHC-FoFr, IHC - Wholemount ; Zebrafish . PubMed: 23671620