Anti-Neomycin Phosphotransferase 2 antibody (ab182391)

Overview

  • Product name
    Anti-Neomycin Phosphotransferase 2 antibody
    See all Neomycin Phosphotransferase 2 primary antibodies
  • Description
    Rabbit polyclonal to Neomycin Phosphotransferase 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    Synthetic peptide within Neomycin Phosphotransferase 2. The exact sequence is proprietary. Klebsiella pneumoniae.
    Database link: P00552

Properties

Applications

Our Abpromise guarantee covers the use of ab182391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 29 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
  • Alternative names
    • Aminoglycoside 3' phosphotransferase antibody
    • APH(3') II antibody
    • APH(3')II antibody
    • aphA antibody
    • EC 2.7.1.95 antibody
    • Kan antibody
    • Kanamycin kinase type II antibody
    • Kanamycin kinase, type VIII antibody
    • kanamycin resistance gene antibody
    • Neo antibody
    • Neomycin kanamycin phosphotransferase type II antibody
    • Neomycin kanamycin phosphotransferase type VIII antibody
    • Neomycin phosphotransferase II antibody
    • Neomycine phosphotransferase antibody
    • NPT II antibody
    • NPTII antibody
    see all

References

ab182391 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



Unfortunately, I can confirm that if you are amplifying the Neo gene from pAcGFP N1 sequence you sent me, there is no homology with the immunogen used to raise the ab49975 antibody, so we would not expect to cross react with the expressed pro...

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