Anti-Neomycin Phosphotransferase 2 antibody (ab33595)

Overview

  • Product name
    Anti-Neomycin Phosphotransferase 2 antibody
    See all Neomycin Phosphotransferase 2 primary antibodies
  • Description
    Rabbit polyclonal to Neomycin Phosphotransferase 2
  • Specificity
    ab33595 recognizes bacterial Neomycin phosphotransferase II (~32kD).
  • Tested applications
    Suitable for: ELISA, WB, IHC-Frmore details
  • Species reactivity
    Reacts with bacteria. Not yet tested in other species.
  • Immunogen

    Purified Neomycin phosphotransferase II.

Properties

Applications

Our Abpromise guarantee covers the use of ab33595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/250.
WB 1/250. Predicted molecular weight: 32 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
  • Alternative names
    • Aminoglycoside 3' phosphotransferase antibody
    • APH(3') II antibody
    • APH(3')II antibody
    • aphA antibody
    • EC 2.7.1.95 antibody
    • Kan antibody
    • Kanamycin kinase type II antibody
    • Kanamycin kinase, type VIII antibody
    • kanamycin resistance gene antibody
    • Neo antibody
    • Neomycin kanamycin phosphotransferase type II antibody
    • Neomycin kanamycin phosphotransferase type VIII antibody
    • Neomycin phosphotransferase II antibody
    • Neomycine phosphotransferase antibody
    • NPT II antibody
    • NPTII antibody
    see all

References

This product has been referenced in:
  • Yamaguchi K  et al. Regulation of plasma-membrane-associated sialidase NEU3 gene by Sp1/Sp3 transcription factors. Biochem J 430:107-17 (2010). ChIP ; Human . Read more (PubMed: 20518744) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Mouse Tissue sections (xenograft mouse model)
Specification
xenograft mouse model
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Paraformaldehyde
Username

Mrs. Aleksandra Kotlyarova

Verified customer

Submitted Jun 11 2014

I am very pleased to hear you would like to accept our offer and test ab60018 in ICC-IF

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Thank you for your enquiry.

I can confirm that to our knowledge, ab33595 and ab60018 have not yet been tested in ICC (immunocytochemistry staining of cell culture), IHC-Frozen sections (IHC-Fr) or IHC-Paraffin tissue sections (IHC-P).
...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Monkey Cultured Cells (COS cells transfected with E. coli neomycin phosph)
Specification
COS cells transfected with E. coli neomycin phosph
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1 % Tween-20
Blocking step
Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 31 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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