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Anti-Neomycin Phosphotransferase 2 antibody (ab49975)


  • Product nameAnti-Neomycin Phosphotransferase 2 antibody
    See all Neomycin Phosphotransferase 2 primary antibodies
  • Description
    Rabbit polyclonal to Neomycin Phosphotransferase 2
  • Specificityab49975 recognizes bacterial Neomycin Phosphotransferase 2 expressed in transgenic tobacco plants.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to internal sequence amino acids 133-150 of Escherichia coli Neomycin Phosphotransferase 2

  • Positive control
    • Cytosolic fraction from the leaves of transgenic Nicotiana tabacum expressing E. coli Neomycin Phosphotransferase 2.



Our Abpromise guarantee covers the use of ab49975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 29 kDa.


  • RelevanceNeomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
  • Database links
    • Alternative names
      • Aminoglycoside 3' phosphotransferase antibody
      • APH(3') II antibody
      • APH(3')II antibody
      • aphA antibody
      • EC antibody
      • Kan antibody
      • Kanamycin kinase type II antibody
      • Kanamycin kinase, type VIII antibody
      • kanamycin resistance gene antibody
      • Neo antibody
      • Neomycin kanamycin phosphotransferase type II antibody
      • Neomycin kanamycin phosphotransferase type VIII antibody
      • Neomycin phosphotransferase II antibody
      • Neomycine phosphotransferase antibody
      • NPT II antibody
      • NPTII antibody
      see all

    Anti-Neomycin Phosphotransferase 2 antibody images

    • Anti-Neomycin Phosphotransferase 2 antibody (ab49975) at 1/500 dilution + Whole cell lysate prepared from SW48 cancer cell line at 20 µg

      Goat anti-rabbit conjugated to HRP at 1/2000 dilution
      developed using the ECL technique

      Predicted band size : 29 kDa
      Observed band size : 29 kDa

      Exposure time : 5 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    References for Anti-Neomycin Phosphotransferase 2 antibody (ab49975)

    ab49975 has not yet been referenced specifically in any publications.

    Product Wall

    Unfortunately, I can confirm that if you are amplifying the Neo gene from pAcGFP N1 sequence you sent me, there is no homology with the immunogen used to raise the ab49975 antibody, so we would not expect to cross react with the expressed pro...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Escherichia coli Cell lysate - whole cell (SW48 cancer cell line)
    Loading amount 20 µg
    Specification SW48 cancer cell line
    Gel Running Conditions Reduced Denaturing (gradient 4-12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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    Submitted Oct 22 2010