Overview

  • Product name
  • Description
    Rabbit polyclonal to Nephrin
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to an internal region of Human Nephrin.

  • Positive control
    • Human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab92887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
    • Tissue specificity
      Specifically expressed in podocytes of kidney glomeruli.
    • Involvement in disease
      Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily.
      Contains 1 fibronectin type-III domain.
      Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
    • Developmental stage
      In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues.
    • Cellular localization
      Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CNF antibody
      • Nephrin antibody
      • Nephrosis 1 congenital Finnish type antibody
      • Nephrosis 1, congenital, Finnish type (nephrin) antibody
      • NPHN antibody
      • NPHN_HUMAN antibody
      • NPHS 1 antibody
      • Nphs1 antibody
      • Renal glomerulus specific cell adhesion receptor antibody
      • Renal glomerulus-specific cell adhesion receptor antibody
      see all

    Images

    • ab92887, at 1/50 dilution staining Nephrin in formalin-fixed, paraffin-embedded Human kidney tissue.

    References

    ab92887 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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