Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)

Overview

  • Product name
    Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1]
    See all Nephrin primary antibodies
  • Description
    Rabbit monoclonal [EPTPG1] to Nephrin (phospho Y1176 + Y1193)
  • Specificity
    This antibody detects Nephrin when either tyrosines 1176 or 1193 are phosphorylated, but does not detect non-phosphorylated Nephrin.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding tyrosine 1176 and 1193 of mouse Nephrin.

  • Positive control
    • Transfected HEK293T cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab80299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 138, 180 kDa (predicted molecular weight: 138 kDa).

Target

  • Function
    Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
  • Tissue specificity
    Specifically expressed in podocytes of kidney glomeruli.
  • Involvement in disease
    Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily.
    Contains 1 fibronectin type-III domain.
    Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
  • Developmental stage
    In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues.
  • Cellular localization
    Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CNF antibody
    • Nephrin antibody
    • Nephrosis 1 congenital Finnish type antibody
    • Nephrosis 1, congenital, Finnish type (nephrin) antibody
    • NPHN antibody
    • NPHN_HUMAN antibody
    • NPHS 1 antibody
    • Nphs1 antibody
    • Renal glomerulus specific cell adhesion receptor antibody
    • Renal glomerulus-specific cell adhesion receptor antibody
    see all

Images

  • All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/50000 dilution

    Lane 1 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin expression vector, whole cell lysate
    Lane 2 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
    Lane 3 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1176F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
    Lane 4 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1193F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 138 kDa
    Observed band size : 138,180 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Jeon J  et al. Dynamin-mediated Nephrin Phosphorylation Regulates Glucose-stimulated Insulin Release in Pancreatic Beta Cells. J Biol Chem 287:28932-42 (2012). WB ; Human . Read more (PubMed: 22718751) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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