Anti-nephronectin antibody (ab110230)

Overview

  • Product name
    Anti-nephronectin antibody
    See all nephronectin primary antibodies
  • Description
    Rabbit polyclonal to nephronectin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human nephronectin.

  • Positive control
    • This antibody gave a positive signal in both Human Kidney and Lung tissue lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).

Target

  • Function
    Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins.
  • Tissue specificity
    Expressed in kidney and lung and to a lower extent in brain, pregnant uterus, placenta, thyroid gland and blood vessels.
  • Sequence similarities
    Belongs to the nephronectin family.
    Contains 5 EGF-like domains.
    Contains 1 MAM domain.
  • Developmental stage
    Expressed in fetal kidney, cochlea, eye, heart and lung.
  • Domain
    The MAM domain is required for localization at the cell surface.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix. Trapped on the cell surface or in the extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • EGFL6L antibody
    • Nephronectin antibody
    • NPNT antibody
    • NPNT_HUMAN antibody
    • POEM antibody
    • Preosteoblast EGF like repeat protein with MAM domain antibody
    • Preosteoblast EGF-like repeat protein with MAM domain antibody
    • Protein EGFL6 like antibody
    • Protein EGFL6-like antibody
    see all

Images

  • All lanes : Anti-nephronectin antibody (ab110230) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References

ab110230 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse)
Gel Running Conditions
Non-reduced Denaturing (4-15%)
Loading amount
400 µg
Specification
Mouse
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Jun

Verified customer

Submitted Jan 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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