Anti-Neprilysin-like Protease beta antibody (ab81688)

Overview

  • Product nameAnti-Neprilysin-like Protease beta antibody
    See all Neprilysin-like Protease beta primary antibodies
  • Description
    Rabbit polyclonal to Neprilysin-like Protease beta
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 371-420 (EVVVYGIPYL QNLENIIDTY SARTIQNYLV WRLVLDRIGS LSQRFKDTRV) of human Neprilysin-like Protease beta (NP_258428).

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

Target

  • RelevanceNeprilysin like Proteases (alpha, beta, gamma) arise from the alternative splicing of a single Neprilysin like Protease gene and show ~54% sequence homology. M13 members are generally type II transmembrane proteins consisting of a single polypeptide chain with Zinc binding HEXXH motif, a short cytoplasmic tail, a transmembrane segment and an extra cytoplasmic domain containing the enzyme active site. Neprilysin like Protease beta is type II transmembrane enzyme containing a single polypeptide chain of 765aa (~110kD) with cytoplasmic and transmembrane domains and a large extracellular C terminal core containing the peptidase active site. In comparison to Neprilysin like Protease alpha, Neprilysin like Protease beta has an additional and unique sequence of 23 aa (41-63 aa) after the TM domain. There are two splice variants of Neprilysin like Protease beta; a secreted isoform of 126kD containing a 23 aa secretion signal sequence and a membrane associated isoform of 110kD. The secreted isoform is much more glycosylated than the membrane isoform. A number of different sequences for human Neprilysin like Protease beta are reported, with 835, 779, 777, 770, 762 and 753 amino acids in length, having predicted masses of 94.8, 89.4, 89.0, 88.5, 87.2 and 86.2 kDa. The 835 amino acid form has a longer and different carboxytermnius than the other forms. The 753 amino acid form has a 26 residue deletion in the juxtamembrane extracellular region, relative to the other forms, and the 770 form starts at the methionine downstream 10 residues from the other forms. The 762 form uses a different exon in the propeptide domain, and a 17 residue deletion. All of these forms have intact catalytic sites, and are thought to be active enzymes.
  • Cellular localizationMembrane; Single pass type II membrane protein. Secreted.
  • Database links
  • Alternative names
    • Mel transforming oncogene like 1 antibody
    • Membrane metallo endopeptidase like 1 antibody
    • Membrane metallo endopeptidase like 2 antibody
    • MMEL1 antibody
    • Neprilysin 2 antibody
    • Neprilysin Like 1 antibody
    • NL1 antibody
    • NL2 antibody
    • SEP antibody
    • Soluble secreted endopeptidase antibody
    see all

Anti-Neprilysin-like Protease beta antibody images

  • Anti-Neprilysin-like Protease beta antibody (ab81688) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa

References for Anti-Neprilysin-like Protease beta antibody (ab81688)

ab81688 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Rat Tissue lysate - whole (Testis and Brain)
Specification Testis and Brain
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Renato Watanabe de Oliveira

Verified customer

Submitted Oct 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"