Recombinant
RabMAb

Anti-Nesprin 1 antibody [EPR14196] (ab192234)

Overview

  • Product name
    Anti-Nesprin 1 antibody [EPR14196]
    See all Nesprin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14196] to Nesprin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 8250-8350. The exact sequence is proprietary.
    Database link: Q8NF91

  • Positive control
    • HeLa, 293, HACAT, RAW 264.7, PC12 and NIH 3T3 cell lysates; human kidney, human bladder transitional cell carcinoma and rat stomach tissues; A673 and HACAT cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14196
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
WB 1/1000 - 1/20000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Required for centrosome migration to the apical cell surface during early ciliogenesis.
  • Tissue specificity
    Widely expressed. Highly expressed in skeletal and smooth muscles, heart, spleen, and peripheral blood leukocytes.
  • Involvement in disease
    Defects in SYNE1 are the cause of spinocerebellar ataxia autosomal recessive type 8 (SCAR8) [MIM:610743]; also known as autosomal recessive cerebellar ataxia type 1 (ARCA1) or recessive ataxia of Beauce. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR8 is an autosomal recessive form.
    Defects in SYNE1 are the cause of Emery-Dreifuss muscular dystrophy type 4 (EDMD4) [MIM:612998]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows, Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects.
  • Sequence similarities
    Belongs to the nesprin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 12 HAT repeats.
    Contains 1 KASH domain.
    Contains 31 spectrin repeats.
  • Domain
    The KASH domain, which contains a transmembrane domain, mediates the nuclear envelope targeting and is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
  • Cellular localization
    Nucleus outer membrane. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere. The largest part of the protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. In skeletal and smooth muscles, a significant amount is found in the sarcomeres.
  • Information by UniProt
  • Database links
  • Alternative names
    • 8B antibody
    • ARCA1 antibody
    • C6orf98 antibody
    • CPG2 antibody
    • CPG2 full length antibody
    • dJ45H2.2 antibody
    • EDMD4 antibody
    • Enaptin antibody
    • Myne-1 antibody
    • MYNE1 antibody
    • Myocyte nuclear envelope protein 1 antibody
    • Nesp1 antibody
    • Nesprin-1 antibody
    • Nuclear envelope spectrin repeat protein 1 antibody
    • SCAR8 antibody
    • Spectrin repeat containing nuclear envelope 1 antibody
    • Synaptic nuclear envelope protein 1 antibody
    • Synaptic nuclei expressed gene 1 antibody
    • Syne-1 antibody
    • SYNE1 antibody
    • SYNE1_HUMAN antibody
    • SYNE1B antibody
    see all

Images

  • Flow cytometric analysis of HACAT cells (paraformaldehyde-fixed, 2%) labeling Nesprin 1 with ab192234 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunofluorescent analysis of A673 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Nesprin 1 with ab192234 at 1/50 dilution (4.3μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution; counter-stained with DAPI (blue).
    Negative controls: anti-Nesprin 1 at 1/50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

  • Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Nesprin 1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

  • Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Nesprin 1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Nesprin 1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

  • All lanes : Anti-Nesprin 1 antibody [EPR14196] (ab192234) at 1/1000 dilution

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 112 kDa

  • All lanes : Anti-Nesprin 1 antibody [EPR14196] (ab192234) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HACAT cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 112 kDa

References

ab192234 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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