Overview

  • Product name
  • Description
    Rabbit polyclonal to Nesprin3
  • Specificity
    This antibody detects endogenous levels of total Nesprin3 protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthesized peptide derived from an internal region of human Nesprin3.

  • Positive control
    • Extracts from Jurkat cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitopespecific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 112 kDa.
ELISA 1/40000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
  • Sequence similarities
    Belongs to the nesprin family.
    Contains 1 KASH domain.
    Contains 2 spectrin repeats.
  • Domain
    The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
  • Cellular localization
    Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf49 antibody
    • Chromosome 14 open reading frame 49 antibody
    • FLJ25605 antibody
    • MGC75267 antibody
    • Nesp3 antibody
    • Nesprin 3 antibody
    • Nesprin-3 antibody
    • NET53 antibody
    • Nuclear Envelope Spectrin Repeat Protein 3 antibody
    • Spectrin Repeat Containing, Nuclear Envelope Family Member 3 antibody
    • SYNE3 antibody
    • SYNE3_HUMAN antibody
    see all

Images

  • All lanes : Anti-Nesprin3 antibody (ab74261) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 112 kDa
    Observed band size : 112 kDa
  • ICC/IF image of ab74261 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74261, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Coffinier C  et al. Abnormal development of the cerebral cortex and cerebellum in the setting of lamin B2 deficiency. Proc Natl Acad Sci U S A 107:5076-81 (2010). WB, ICC/IF ; Mouse . Read more (PubMed: 20145110) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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