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Nestin-transfected NS0 cells transfected with a human Nestin fragment aa residues 618-1618.
Our Abpromise guarantee covers the use of ab6320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 0.1 µg/ml. When using aldehyde-based fixative, use at a concentration of 5 µg/ml (see Abreview).|
|IHC - Wholemount||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC||Use a concentration of 5 - 10 µg/ml. Tested in human neural progenitors and A172 cells. Cells were fixed with 4% paraformaldehyde and 0.15% picric acid in PBS at RT for 20 min.|
|Flow Cyt||Use a concentration of 25 µg/ml.
Fix and permeabilize cells.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Immunocytochemical analysis of human fetal neural progenitor cells, labeling Nestin with ab6320 (10µg/ml). Immunostaining was for 3 hours at room temperature. Counterstaining with DAPI (blue).