Overview

  • Product nameAnti-Nestin antibody [2C1.3A11]See all Nestin primary antibodies ...
  • Description
    Mouse monoclonal [2C1.3A11] to Nestin
  • Tested applicationsWB, ICC, Flow Cyt, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Rat
  • Immunogen

    A 150 amino acid fragment from the cloned human Nestin.

  • General notes


    This highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab18102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 177 kDa.
ICC 1/100 - 1/500.
Flow Cyt 1/200.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificityCNS stem cells.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageUpon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • Nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody [2C1.3A11] images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of migrating tumor cells labelling Nestin with ab18102 at 2µg/ml. Cytoplasm and membrane positive staining shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of xenograft tumor cells labelling Nestin with ab18102.

  • All lanes : Anti-Nestin antibody [2C1.3A11] (ab18102) at 1/1000 dilution

    Lane 1 : Total rat brain protein at 5 µg
    Lane 2 : Human CNS progenitor cell protein at 1 µg
    Lane 3 : Human CNS progenitor cell protein at 5 µg


    Predicted band size : 177 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab18102 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18102, 1/200 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry of Anti-Nestin on formalin fixed paraffin embedded prostate nerve tissue using ab18102
  • Immunofluorescence staining of U251 cells (glioblastoma cell line) stained using ab18102(green) and bisbenzimide (blue)

References for Anti-Nestin antibody [2C1.3A11] (ab18102)

This product has been referenced in:
  • Sherry MM  et al. STAT3 is required for proliferation and maintenance of multipotency in glioblastoma stem cells. Stem Cells 27:2383-92 (2009). WB ; Human . Read more (PubMed: 19658181) »
  • Messam CA  et al. Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody. Exp Neurol 161:585-96 (2000). Read more (PubMed: 10686078) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Sample Human Cell (Neural Progenitor cells (from human ES cells))
Specification Neural Progenitor cells (from human ES cells)
Preparation Cell harvesting/tissue preparation method: Cell Dissociation Buffer
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation Paraformaldehyde
Permeabilization Yes - 70% Methanol (ice cold)
Gating Strategy FSC vs SSC on negative control sample
Username

Abcam user community

Verified customer

Submitted Jan 03 2012

I am please to be able to share the following protocol for ab18102for Flow cytometry: Fix cells with 4% Paraformaldehyde for 30 min at room temperature (RT) Wash twice with 1% PBS at 1500rpm/5min/RT Permeabilize cells with 0.02% Triton X-100 in 1%P...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"