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Nestin-transfected NS0 cells transfected with a human Nestin fragment aa residues 618-1618.
Our Abpromise guarantee covers the use of ab6320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 0.1 µg/ml. When using aldehyde-based fixative, use at a concentration of 5 µg/ml (see Abreview).|
|IHC - Wholemount||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC||Use a concentration of 5 - 10 µg/ml. Tested in human neural progenitors and A172 cells. Cells were fixed with 4% paraformaldehyde and 0.15% picric acid in PBS at RT for 20 min.|
|Flow Cyt||Use a concentration of 25 µg/ml.
Fix and permeabilize cells.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 200 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab6320 staining Nestin in human iCell® Neurons by Flow Cytometry. Cells were harvested in 1 mM EDTA/1 mM EGTA in PBS (calcium- and magnesium-free), fixed with acetone and permeabilized with 0.25% Triton X-100 in DPBS. The sample was incubated with the primary antibody (1/300 in 10% FBS) for 2 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Gating Strategy: negative control cells.