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Does not react withMouse, Rat
Nestin-transfected NS0 cells transfected with a human Nestin fragment aa residues 618-1618.
Our Abpromise guarantee covers the use of ab6320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 0.1 µg/ml. When using aldehyde-based fixative, use at a concentration of 5 µg/ml (see Abreview).|
|IHC - Wholemount||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC||Use a concentration of 5 - 10 µg/ml. Tested in human neural progenitors and A172 cells. Cells were fixed with 4% paraformaldehyde and 0.15% picric acid in PBS at RT for 20 min.|
|Flow Cyt||Use a concentration of 25 µg/ml. Fix and permeabilize cells.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 200 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab6320 staining Nestin in human LGE neural progenitors by Flow Cytometry. Cells were paraformaldehyde and permeabilized. The sample was incubated with the primary antibody (1/200) for 1 hour at 20°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG1 (1/800) was used as the secondary antibody.
Gating Strategy: Isotype population (shown in white).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"