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Does not react withHuman
Nestin purified from embryonic rat spinal cord.
Our Abpromise guarantee covers the use of ab11306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 170, 350 kDa (predicted molecular weight: 177 kDa). The use of BSA as blocking agent is not recommended.|
|IHC-P||Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab11306 staining Nestin in rat lateral ventricle (subventricular zone) sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 1% BSA for 10 minutes at room temperature. Antigen retrieval was by heat mediation in citric acid (pH6). Samples were incubated with primary antibody 1/200 (TBS/BSA/azide pH7.5) for 24 hours. A Biotin-conjugated Goat polycolonal to mouse IgG, dilution 1/200, was used as secondary antibody. The image shows clear staining in cells of the rat subventricular zone of the lateral ventricle.
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