Overview

  • Product name
  • Description
    Rabbit polyclonal to Nestin
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Nestin (internal sequence).

  • Positive control
    • Human liver carcinoma tissue IF/ICC: SKNSH cells

Properties

Applications

Our Abpromise guarantee covers the use of ab93666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
Flow Cyt 1/250.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. 1/200 for 30 minutes at room temperature. Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target

  • Function
    Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificity
    CNS stem cells.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Developmental stage
    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • Database links
  • Alternative names
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody images

  • ab93666, at a 1/200 dilution, staining Nestin in formalin fixed, paraffin embedded Human liver carcinoma tissue by Immunohistochemistry.
  • ICC/IF image of ab93666 stained SKNSH cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93666, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Nestin antibody (ab93666)

This product has been referenced in:
  • Miyazawa K  et al. Immunohistochemical expression of four different stem cell markers in prostate cancer: High expression of NANOG in conjunction with hypoxia-inducible factor-1a expression is involved in prostate epithelial malignancy. Oncol Lett 8:985-992 (2014). IHC-P ; Human . Read more (PubMed: 25120646) »
  • Nogueira AB  et al. Existence of a potential neurogenic system in the adult human brain. J Transl Med 12:75 (2014). IHC-P ; Human . Read more (PubMed: 24655332) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (neural stem cell)
Specification
neural stem cell
Fixation
BD cytofix-cytoperm
Permeabilization
Yes - BD cytofix-cytoperm
Gating Strategy
Doublets were excluded
Username

Abcam user community

Verified customer

Submitted Jan 16 2012

DISCOUNT CODE: ******** Expiration date: ******** I am very pleased to hear you would like to accept our offer and test ab93666 in FACS. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, p...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up