Overview

  • Product name
  • Description
    Rabbit polyclonal to NET1
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 546-596 of Human NET1 (NP_001040625).

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab113202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHG8_HUMAN antibody
    • ARHGEF8 antibody
    • GLYT2 antibody
    • Guanine nucleotide regulatory protein oncogene antibody
    • MGC114559 antibody
    • MGC127767 antibody
    • MGC53602 antibody
    • mNET1 antibody
    • Net1 antibody
    • NET1A antibody
    • Neuroepithelial cell transforming gene 1 antibody
    • Neuroepithelial cell-transforming gene 1 protein antibody
    • p65 Net1 proto oncogene antibody
    • Proto-oncogene p65 Net1 antibody
    • Rho guanine nucleotide exchange factor 8 antibody
    • Rho guanine nucleotide exchange factor GEF 8 antibody
    see all

Images

  • ab113202 staining NET1 in PC-12 cells treated with milnacipran hydrochloride (ab120755), by ICC/IF. Decrease of NET1 expression correlates with increased concentration of milnacipran hydrochloride, as described in literature.
    The NGF treated cells were incubated at 37°C for 6 hour in media containing different concentrations of ab120755 (milnacipran hydrochloride ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab113202 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • All lanes : Anti-NET1 antibody (ab113202) at 0.4 µg/ml

    Lane 1 : Jurkat Cell lysate at 50 µg
    Lane 2 : Jurkat Cell lysate at 15 µg
    Lane 3 : Jurkat Cell lysate at 5 µg


    Predicted band size : 62 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of NET1 (6 µg of antibody per mg of Jurkat cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-Net1 antibody that recognizes an upstream epitope; Lane 2- Immunoprecipitation with ab113202; Lane 3- Immunoprecipitation with IgG control. Anti-Net 1 antibody (ab113202) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 3 min.

References

ab113202 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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