Overview

  • Product name
  • Description
    Rabbit polyclonal to NETO1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C-terminal amino acids 303-352 (MCINNTLVCN GLQNCVYPWD ENHCKEKRKT SLLDQLTNTS GTVIGVTSCI) of Human NETO1 (NP_620416).

  • Positive control
    • 721_B cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.
  • Tissue specificity
    Isoform 1 and isoform 2 are retina-specific. Isoform 3 is found in retina as well as at lower levels in adult and fetal brain.
  • Sequence similarities
    Contains 2 CUB domains.
    Contains 1 LDL-receptor class A domain.
  • Cellular localization
    Secreted; Cell membrane and Cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Component of the postsynaptic density (PSD) of excitatory synapses.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI851453 antibody
    • BCTL1 antibody
    • Brain specific transmembrane protein containing 2 CUB and 1 LDL receptor class A domains protein 1 antibody
    • Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 antibody
    • BTCL1 antibody
    • C130005O10Rik antibody
    • FLJ41325 antibody
    • Neto1 antibody
    • NETO1_HUMAN antibody
    • Neuropilin and tolloid like 1 antibody
    • Neuropilin and tolloid-like protein 1 antibody
    • RGD1566269 antibody
    see all

Images

  • Immunohistochemistry with mice retinal neurons tissue using anti-NETO1 antibody (ab111374).

  • Anti-NETO1 antibody (ab111374) at 1 µg/ml + 721_B cell lysate at 10 µg

    Predicted band size : 58 kDa
    Gel concentration: 12%.

References

ab111374 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
10 cells
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Recombinant protein (X. laevis oocytes)
Specification
X. laevis oocytes
Treatment
ConA-purified
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Nov 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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