Overview

  • Product nameAnti-NETO1 antibody
    See all NETO1 primary antibodies
  • Description
    Rabbit polyclonal to NETO1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C-terminal amino acids 303-352 (MCINNTLVCN GLQNCVYPWD ENHCKEKRKT SLLDQLTNTS GTVIGVTSCI) of Human NETO1 (NP_620416).

  • Positive control
    • 721_B cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInvolved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.
  • Tissue specificityIsoform 1 and isoform 2 are retina-specific. Isoform 3 is found in retina as well as at lower levels in adult and fetal brain.
  • Sequence similaritiesContains 2 CUB domains.
    Contains 1 LDL-receptor class A domain.
  • Cellular localizationSecreted; Cell membrane and Cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Component of the postsynaptic density (PSD) of excitatory synapses.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI851453 antibody
    • BCTL1 antibody
    • Brain specific transmembrane protein containing 2 CUB and 1 LDL receptor class A domains protein 1 antibody
    • Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 antibody
    • BTCL1 antibody
    • C130005O10Rik antibody
    • FLJ41325 antibody
    • Neto1 antibody
    • NETO1_HUMAN antibody
    • Neuropilin and tolloid like 1 antibody
    • Neuropilin and tolloid-like protein 1 antibody
    • RGD1566269 antibody
    see all

Anti-NETO1 antibody images

  • Immunohistochemistry with mice retinal neurons tissue using anti-NETO1 antibody (ab111374).

  • Anti-NETO1 antibody (ab111374) at 1 µg/ml + 721_B cell lysate at 10 µg

    Predicted band size : 58 kDa
    Gel concentration: 12%.

References for Anti-NETO1 antibody (ab111374)

ab111374 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 10 cells
Gel Running Conditions Reduced Denaturing (10)
Sample Rat Recombinant protein (X. laevis oocytes)
Specification X. laevis oocytes
Treatment ConA-purified
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Nov 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"