Overview

  • Product name
  • Description
    Rabbit polyclonal to NETO2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (GIKHIPATQC GIWVRTSNGG HFASPNYPDS YPPNKECIYI LEAAPRQRIE) of human NETO2 (NP_060562).

  • Positive control
    • 721_B cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3. Increases kainate-receptor channel activity, slowing the decay kinetics of the receptors, without affecting their expression at the cell surface, and increasing the open probability of the receptor channels. Modulates the agonist sensitivity of kainate receptors. Slows the decay of kainate receptor-mediated excitatory postsynaptic currents (EPSCs), thus directly influencing synaptic transmission.
  • Sequence similarities
    Contains 2 CUB domains.
    Contains 1 LDL-receptor class A domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain specific transmembrane protein antibody
    • Brain specific transmembrane protein containing 2 CUB and 1 LDL receptor class A domains protein 2 antibody
    • Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2 antibody
    • BTCL2 antibody
    • FLJ10430 antibody
    • FLJ14724 antibody
    • FLJ90456 antibody
    • NEOT2 antibody
    • NETO 2 antibody
    • Neto2 antibody
    • NETO2_HUMAN antibody
    • Neuropilin (NRP) and tolloid (TLL) like 2 antibody
    • Neuropilin and tolloid like protein 2 antibody
    • Neuropilin and tolloid-like protein 2 antibody
    see all

Anti-NETO2 antibody images

  • Anti-NETO2 antibody (ab82651) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NETO2 antibody (ab82651)

ab82651 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Loading amount
10 cells
Gel Running Conditions
Reduced Denaturing (10 %)
Sample
Rat Recombinant protein (X. laevis oocytes)
Specification
X. laevis oocytes
Treatment
ConA-purified
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Nov 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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