Overview

  • Product name
    Anti-NETO2 antibody - N-terminal
    See all NETO2 primary antibodies
  • Description
    Rabbit polyclonal to NETO2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NETO2 aa 173-203 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. (NP_060562.3).
    Database link: Q8NC67

  • Positive control
    • Jurkat cell line lysate; Human colon carcinoma tissue; Jurkat cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171651 is purified through a Protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 59 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3. Increases kainate-receptor channel activity, slowing the decay kinetics of the receptors, without affecting their expression at the cell surface, and increasing the open probability of the receptor channels. Modulates the agonist sensitivity of kainate receptors. Slows the decay of kainate receptor-mediated excitatory postsynaptic currents (EPSCs), thus directly influencing synaptic transmission.
  • Sequence similarities
    Contains 2 CUB domains.
    Contains 1 LDL-receptor class A domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain specific transmembrane protein antibody
    • Brain specific transmembrane protein containing 2 CUB and 1 LDL receptor class A domains protein 2 antibody
    • Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2 antibody
    • BTCL2 antibody
    • FLJ10430 antibody
    • FLJ14724 antibody
    • FLJ90456 antibody
    • NEOT2 antibody
    • NETO 2 antibody
    • Neto2 antibody
    • NETO2_HUMAN antibody
    • Neuropilin (NRP) and tolloid (TLL) like 2 antibody
    • Neuropilin and tolloid like protein 2 antibody
    • Neuropilin and tolloid-like protein 2 antibody
    see all

Images

  • Anti-NETO2 antibody - N-terminal (ab171651) at 1/2000 dilution (Anti-NETO2 Antibody (N-term )) + Jurkat whole cell lysate at 20 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugated

    Predicted band size: 59 kDa
    Observed band size: 110,57 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-NETO2 antibody - N-terminal (ab171651) at 1 µg/ml

    Lane 1 : Rat whole brain lysate (P31) at 25 µg
    Lane 2 : Mouse whole brain lysate (3mnths) at 25 µg
    Lane 3 : HEK293 transfected with HA-Neto1 plasmid at 25 µg
    Lane 4 : HEK293 transfected with myc-Neto2 plasmid at 25 µl
    Lane 5 : non-transfected HEK293 at 25 µg

    Secondary
    All lanes : HRP conjugated goat anti-rabbit at 1/8000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 110 kDa (possible non-specific binding), 55 kDa (possible non-specific binding)


    Exposure time: 3 minutes


    ab171651 detecting Neto2 in mouse and rat brain lysates (lanes 1 and 2) as indicated by arrow on left hand side. ab171651 detects Neto2 in HEK293 cells tranfected with myc tagged Neto2 (lane 4), however it does not bind unespecifically Neto1 in HEK293 cells tranfected with HA tagged Neto1 (lane 3). For experimental details please see abreview.

    See Abreview

  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling NETO2 with ab171651 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometric analysis of Jurkat cells with ab171651 antibody at a dilution of 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated Donkey-anti-Rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:
  • Hu L  et al. Upregulation of NETO2 expression correlates with tumor progression and poor prognosis in colorectal carcinoma. BMC Cancer 15:1006 (2015). Read more (PubMed: 26699544) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Dr. Garry Whitehead

Verified customer

Submitted Sep 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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