Recombinant Anti-Netrin 1 antibody [EPR5428] (ab126729)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5428] to Netrin 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Netrin 1 antibody [EPR5428]
See all Netrin 1 primary antibodies -
Description
Rabbit monoclonal [EPR5428] to Netrin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab192272) -
Positive control
- WB: Human fetal brain, human small intestine, mouse kidney, mouse heart, rat kidney and brain tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5428 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Mouse heart tissue lysate - total protein (0 days) (ab7193)
- Mouse heart tissue lysate - total protein (14 days) (ab7194)
- Mouse kidney tissue lysate - total protein (0 days) (ab7261)
- Mouse kidney tissue lysate (14 days) (ab7262)
- Mouse kidney tissue lysate (7 days) (ab7263)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/500 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
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Notes |
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WB
1/500 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis. -
Tissue specificity
Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. -
Sequence similarities
Contains 3 laminin EGF-like domains.
Contains 1 laminin N-terminal domain.
Contains 1 NTR domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 9423 Human
- Entrez Gene: 18208 Mouse
- Entrez Gene: 114523 Rat
- Omim: 601614 Human
- SwissProt: O95631 Human
- SwissProt: O09118 Mouse
- SwissProt: Q924Z9 Rat
- Unigene: 660885 Human
see all -
Alternative names
- epididymis tissue protein Li 131P antibody
- NET1_HUMAN antibody
- Netrin 1, mouse, homolog of antibody
see all
Images
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All lanes : Anti-Netrin 1 antibody [EPR5428] (ab126729) at 1/500 dilution
Lane 1 : Rat brain lysates prepared in RIPA lysis method
Lane 2 : Rat brain lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-Netrin 1 antibody [EPR5428] (ab126729) at 1/1000 dilution (purified)
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 67 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-Netrin 1 antibody [EPR5428] (ab126729) at 1/1000 dilution (purified) + Human fetal brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 67 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Netrin 1 antibody [EPR5428] (ab126729) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal brain lysate
Lane 2 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 67 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab126729 has been referenced in 40 publications.
- Jiang RC et al. CD146 mediates the anti-apoptotic role of Netrin-1 in endothelial progenitor cells under hypoxic conditions. Mol Med Rep 25:N/A (2022). PubMed: 34738629
- Jiang W et al. PGE2 activates EP4 in subchondral bone osteoclasts to regulate osteoarthritis. Bone Res 10:27 (2022). PubMed: 35260562
- Ma L et al. Neural Stem Cell-Derived Exosomal Netrin1 Contributes to Neuron Differentiation of Mesenchymal Stem Cells in Therapy of Spinal Bifida Aperta. Stem Cells Transl Med 11:539-551 (2022). PubMed: 35325230
- Feng XF et al. Magnetic Resonance Imaging Investigation of Neuroplasticity After Ischemic Stroke in Tetramethylpyrazine-Treated Rats. Front Pharmacol 13:851746 (2022). PubMed: 35559236
- Yu C et al. Corneal Epithelium-Derived Netrin-1 Alleviates Dry Eye Disease via Regulating Dendritic Cell Activation. Invest Ophthalmol Vis Sci 63:1 (2022). PubMed: 35648640