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Rabbit polyclonal to Netrin G1 ligand
Predicted to work with:
Mouse, Rat, Zebrafish
Recombinant fragment, corresponding to a region within amino acids 5-195 of Human Netrin G1 ligand.
NT2D1, IMR32, U-87 MG and MCF7 cells and whole cell lysates; Human breast cancer tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 72 kDa.
1/100 - 1/500.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: heat mediated Tris-EDTA buffer, pH 8.0
May promote neurite outgrowth of developing thalamic neurons.
Highly expressed in the cerebral cortex, including frontal, parietal and occipital lobes. Putamen, amygdala, hippocampus and medulla oblongata show moderate expression. Caudate nucleus and thalamus express small amounts, whereas other brain regions show very weak or no expression.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 9 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.
The LRR region is both necessary and sufficient for the interaction with NTNG1.
Cell junction, synapse, postsynaptic cell membrane.
Information by UniProt
Leucine rich repeat containing 4C antibody
Western blot - Netrin G1 ligand antibody (ab111572)
Anti-Netrin G1 ligand antibody (ab111572) at 1/2000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 72 kDa 7.5% SDS-PAGE.
Immunocytochemistry/ Immunofluorescence - Netrin G1 ligand antibody (ab111572)
ab111572 at 1/500 dilution staining Netrin G1 ligand in methanol-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Netrin G1 ligand antibody (ab111572)
Paraffin-embedded human breast cancer tissue stained for Netrin G1 ligand using ab111572 at 1/250 dilution in Immunohistochemical analysis.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"