Anti-Netrin G1 ligand antibody (ab111572)

Overview

  • Product name
    Anti-Netrin G1 ligand antibody
  • Description
    Rabbit polyclonal to Netrin G1 ligand
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 5-195 of Human Netrin G1 ligand.

  • Positive control
    • NT2D1, IMR32, U-87 MG and MCF7 cells and whole cell lysates; Human breast cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.
ICC/IF 1/100 - 1/500.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: heat mediated Tris-EDTA buffer, pH 8.0

Target

  • Function
    May promote neurite outgrowth of developing thalamic neurons.
  • Tissue specificity
    Highly expressed in the cerebral cortex, including frontal, parietal and occipital lobes. Putamen, amygdala, hippocampus and medulla oblongata show moderate expression. Caudate nucleus and thalamus express small amounts, whereas other brain regions show very weak or no expression.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Domain
    The LRR region is both necessary and sufficient for the interaction with NTNG1.
  • Cellular localization
    Cell junction, synapse, postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6430556C10Rik antibody
    • KIAA1580 antibody
    • Leucine rich repeat containing 4C antibody
    • Leucine-rich repeat-containing protein 4C antibody
    • LRC4C_HUMAN antibody
    • LRRC4C antibody
    • mKIAA1580 antibody
    • Netrin-G1 ligand antibody
    • NGL 1 antibody
    • NGL-1 antibody
    • NGL1 antibody
    • RGD1311013 antibody
    see all

Images

  • Anti-Netrin G1 ligand antibody (ab111572) at 1/2000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size : 72 kDa
    7.5% SDS-PAGE.
  • ab111572 at 1/500 dilution staining Netrin G1 ligand in methanol-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
  • Paraffin-embedded human breast cancer tissue stained for Netrin G1 ligand using ab111572 at 1/250 dilution in Immunohistochemical analysis.

References

ab111572 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Human Cell (MCF-7)
Permeabilization
No
Specification
MCF-7
Preparation
Cell harvesting/tissue preparation method: harvest using trypsin
Sample buffer: no
Username

Miss. Hadas Pahima

Verified customer

Submitted Jul 17 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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