Overview

  • Product nameAnti-Netrin G1 ligand antibody
    See all Netrin G1 ligand primary antibodies
  • Description
    Rabbit polyclonal to Netrin G1 ligand
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (LVVAGLVRAQ TCPSVCSCSN QFSKVICVRK NLREVPDGIS TNTRLLNLHE) of Human Netrin G1 ligand (NP_065980).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay promote neurite outgrowth of developing thalamic neurons.
  • Tissue specificityHighly expressed in the cerebral cortex, including frontal, parietal and occipital lobes. Putamen, amygdala, hippocampus and medulla oblongata show moderate expression. Caudate nucleus and thalamus express small amounts, whereas other brain regions show very weak or no expression.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • DomainThe LRR region is both necessary and sufficient for the interaction with NTNG1.
  • Cellular localizationCell junction, synapse, postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6430556C10Rik antibody
    • KIAA1580 antibody
    • Leucine rich repeat containing 4C antibody
    • Leucine-rich repeat-containing protein 4C antibody
    • LRC4C_HUMAN antibody
    • LRRC4C antibody
    • mKIAA1580 antibody
    • Netrin-G1 ligand antibody
    • NGL 1 antibody
    • NGL-1 antibody
    • NGL1 antibody
    • RGD1311013 antibody
    see all

Anti-Netrin G1 ligand antibody images

  • Anti-Netrin G1 ligand antibody (ab83001) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 71 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References for Anti-Netrin G1 ligand antibody (ab83001)

ab83001 has not yet been referenced specifically in any publications.

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