Recombinant
RabMAb

Anti-NeuN antibody [EPR12763] (Biotin) (ab204681)

Overview

  • Product nameAnti-NeuN antibody [EPR12763] (Biotin)
    See all NeuN primary antibodies
  • Description
    Rabbit monoclonal [EPR12763] to NeuN (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset
  • Immunogen

    Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: A6NFN3

  • Positive control
    • IHC-P: FFPE human cerebellum (normal) tissue sections.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:
    Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Anti-NeuN antibody (Alexa Fluor® 488) [EPR12763] - Neuronal Marker (ab190195)

    Anti-NeuN antibody (Alexa Fluor® 568) [EPR12763] - Neuronal Marker (ab207282)

    Anti-NeuN antibody (Alexa Fluor® 647) [EPR12763] - Neuronal Marker (ab190565)

Properties

Applications

Our Abpromise guarantee covers the use of ab204681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-NeuN antibody [EPR12763] (Biotin) images

  • IHC image of NeuN staining in a section of formalin-fixed paraffin-embedded human cerebellum (normal), performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin. The section was then incubated with ab204681, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-NeuN antibody [EPR12763] (Biotin) (ab204681)

ab204681 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"