Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-NeuN antibody [EPR12763] - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.5% Tween
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/Tween/azide
Dilution
1/6000

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000

Additional data

Additional Notes
All areas of the brain gave strong specific neuron positivity. Submitted composite image illustrates this: in cerebellum ( upper image) and Pons region ( lower image).
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 08 2014

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