Recombinant
RabMAb

Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 568) (ab207282)

Overview

  • Product nameAnti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 568)
    See all NeuN primary antibodies
  • Description
    Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker (Alexa Fluor® 568)
  • ConjugationAlexa Fluor® 568. Ex: 578nm, Em: 603nm
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Sheep, Goat, Cat, Dog, Human, Zebrafish, Common marmoset
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: A6NFN3

  • Positive control
    • ICC/IF: PC12 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
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    Alternative versions available:

    Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Anti-NeuN antibody (Alexa Fluor® 488) [EPR12763] - Neuronal Marker (ab190195)
    Anti-NeuN antibody (Alexa Fluor® 647) [EPR12763] - Neuronal Marker (ab190565)

    Anti-NeuN antibody (Biotin) [EPR12763] (ab204681)

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab207282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in PC12 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 568) images

  • ab207282 staining NeuN in PC12 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207282 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in PC12 cells fixed with 4% formaldehyde (10 min).

References for Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 568) (ab207282)

ab207282 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"