Neuraminidase Activity Assay Kit (Fluorometric) (ab185436)

Overview

  • Product name
    Neuraminidase Activity Assay Kit (Fluorometric)
    See all Neuraminidase kits
  • Detection method
    Fluorescent
  • Sample type
    Serum, Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    < 2 mU/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human, Mammal
  • Product overview

    Abcam's Neuraminidase Activity Assay Kit (Fluorometric) (ab185436) provides a simple and sensitive method for measuring neuraminidase (NA) activity using fluorescence (Ex/Em = 530/590 nm). The assay utilizes NA Probe to detect the neuraminidase activity. This high-throughput adaptable assay kit can detect NA activity as low as 2.0 mU/mL in a variety of samples.

  • Notes

    Neuraminidase (NA) is a very common enzyme that hydrolyzes terminal sialic acid residues on polysaccharide chains; most often a galactose residue. NA activity plays a key role in the invasion of target cells and the replication of influenza virus. NA activity also assists in the elution of progeny viruses from infected cells, and prevents self-aggregation of virus. Thus, NA is an important target for drug development.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Galactose Standard (100nmol/ul) Yellow 1 x 0.1ml
    NA Assay Buffer 1 x 30ml
    NA Enzyme Mix I 1 vial
    NA Enzyme Mix II 1 vial
    NA Positive Control 1 x 0.1ml
    NA Probe 1 x 0.2ml
    NA Substrate 1 vial
  • Research areas
  • Function
    Catalyzes the removal of sialic acid (N-acetylneuramic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.
  • Tissue specificity
    Highly expressed in pancreas, followed by skeletal muscle, kidney, placenta, heart, lung and liver. Weakly expressed in brain.
  • Involvement in disease
    Defects in NEU1 are the cause of sialidosis (SIALIDOSIS) [MIM:256550]. It is a lysosomal storage disease occurring as two types with various manifestations. Type 1 sialidosis (cherry red spot-myoclonus syndrome or normosomatic type) is late-onset and it is characterized by the formation of cherry red macular spots in childhood, progressive debilitating myoclonus, insiduous visual loss and rarely ataxia. The diagnosis can be confirmed by the screening of the urine for sialyloligosaccharides. Type 2 sialidosis (also known as dysmorphic type) occurs as several variants of increasing severity with earlier age of onset. It is characterized by the presence of abnormal somatic features including coarse facies and dysostosis multiplex, vertebral deformities, mental retardation, cherry-red spot/myoclonus, sialuria, cytoplasmic vacuolation of peripheral lymphocytes, bone marrow cells and conjunctival epithelial cells.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 33 family.
    Contains 4 BNR repeats.
  • Domain
    A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.
  • Post-translational
    modifications
    N-glycosylated.
    Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.
  • Cellular localization
    Lysosome membrane. Lysosome lumen. Cell membrane. Cytoplasmic vesicle. Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.
  • Information by UniProt
  • Alternative names
    • Acetylneuraminyl hydrolase
    • G9 sialidase
    • Lysosomal sialidase
    • N-acetyl-alpha-neuraminidase 1
    • NEU1
    • NEUR1_HUMAN
    • Sialidase-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab185436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • NA activity in normal Human serum (1 µl) and positive control (1 µl).

Protocols

References

ab185436 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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