Overview

  • Product nameAnti-Neurexin II alpha antibody
  • Description
    Rabbit polyclonal to Neurexin II alpha
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Rat Neurexin II alpha.

    (Peptide available as ab34449.)

  • Positive control
    • normal adult human cerebral cortex SKNSH cell line

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab34245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/700. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/400.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • RelevanceNeurexin 2 alpha belongs to the Neurexin family, a group of neuronal proteins that function as cell adhesion molecules during synaptogenesis and intracellular signaling. Mammalian genomes contain just three neurexin genes. However, many (>200) neurexin isoforms may be expressed through the use of two alternative promoters and alternative splicing at up to five different positions.
  • Cellular localizationMembrane; single-pass type I membrane protein.
  • Database links
  • Alternative names
    • Neurexin 2 antibody
    • Neurexin II antibody
    • NRXN2 antibody

Anti-Neurexin II alpha antibody images

  • (Image courtesy of Human Protein Atlas)
    ab34245 staining Neurexin II alpha protein in normal adult human cerebral cortex. Brown color indicates presence of protein, blue color shows cell nuclei. Paraffin embedded human cerebral cortex tissue was incubated with ab34245 (1/700 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    .
  • Anti-Neurexin II alpha antibody (ab34245) at 1/400 dilution + whole cell lysate prepared from murine N2A cells at 20 µg

    Secondary
    Goat polyclonal conjugated to Dylight 800 at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 185 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)

    Image courtesy of an anonymous Abreview.

    See Abreview

  • ICC/IF image of ab34245 stained SKNSH cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34245, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Neurexin II alpha antibody (ab34245)

This product has been referenced in:
  • Shapiro-Reznik M  et al. Diurnal rhythms in neurexins transcripts and inhibitory/excitatory synapse scaffold proteins in the biological clock. PLoS One 7:e37894 (2012). WB ; Rat . Read more (PubMed: 22662246) »
  • Borsics T  et al. Subcellular distribution and expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in malignant glioma: Influence on cell survival and migration. Cancer Sci : (2010). IHC-P ; Human . Read more (PubMed: 20412121) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry regarding ab34245 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

The expected band size for mature Neurexin II alp...

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Thank you for your enquiry regarding ab34245.

According to Swiss-Prot database, there seems to be some splice variants and isoforms Neurexin II alpha. You may wish to take a look at this site for further information:

http://www.unip...

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Thank you very much for your interest in ab34245. To our knowledge, ab34245 has not been tested in Rat (it is only predicted). Therefore, I can offer a discount off a future purchase if you buy ab34245 now, test it in Rat and submit feedback to us in t...

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Application Western blot
Sample Mouse Cell lysate - whole cell (N2A cells)
Loading amount 20 µg
Specification N2A cells
Gel Running Conditions Reduced Denaturing
Blocking step casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 37°C
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Verified customer

Submitted Dec 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"