Overview

  • Product nameAnti-NEURL antibody
    See all NEURL primary antibodies
  • Description
    Rabbit polyclonal to NEURL
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of human NEURL.

  • Positive control
    • extracts from MCF-7 cells IF/ICC: MCF7 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab74262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ELISA 1/40000.

Target

  • RelevanceNEURL is involved in the determination of cell fate in the neurogenic region of the embryo and plays a role in the determination of cell fate in the central nervous system. NEURL may act as a tumor suppressor whose inactivation could be associated with malignant progression of astrocytic tumors.
  • Cellular localizationCell membrane; Peripheral membrane protein. Cytoplasm; perinuclear region.
  • Database links
  • Alternative names
    • h neu antibody
    • h neuralized 1 antibody
    • Neu 1 antibody
    • Neu antibody
    • Neuralized homolog antibody
    • Neuralized like protein 1 antibody
    • Neuralized like protein 1A antibody
    • NEURL1 antibody
    • RING finger protein 67 antibody
    • RNF67 antibody
    see all

Anti-NEURL antibody images

  • ICC/IF image of ab74262 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74262, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-NEURL antibody (ab74262) at 1/500 dilution

    Lane 1 : extracts from MCF-7 cells
    Lane 2 : extracts from MCF-7 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 62 kDa
    Observed band size : 62 kDa

References for Anti-NEURL antibody (ab74262)

ab74262 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"