Overview

  • Product name
  • Description
    Rabbit polyclonal to NEURL1B
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: ALIDVYGITD EVQLLESAFA DTLTPARLSQ ARFSACLPPS SHDAANFDNN ELENNQVVAK LGHL, corresponding to amino acids 178-241 of Human NEURL1B according to A8MQ27

  • Positive control
    • Human rectum tissue; Human liver, Human tonsil, RT-4 and U-251 MG cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    E3 ubiquitin-protein ligase.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 2 NHR (neuralized homology repeat) domains.
    Contains 1 RING-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase NEURL1B antibody
    • E3 ubiquitin-protein ligase NEURL3 antibody
    • hNeur2 antibody
    • NEU1B_HUMAN antibody
    • Neur2 antibody
    • neuralized-2 alternative protein antibody
    • neuralized-2 antibody
    • Neuralized-like protein 1B antibody
    • Neuralized-like protein 3 antibody
    • NEURL1B antibody
    • NEURL3 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in vesicles. Recommended concentration of ab122400 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122400, at a 1/225 dilution, staining NEURL1B in glandular cells of paraffin-embedded Human rectum tissue by immunohistochemistry.
  • All lanes : Anti-NEURL1B antibody (ab122400) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab122400 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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