The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesThis antibody gave a positive result in Peptide ELISA against the immunizing peptide. Antibody detection limit dilution 1/8000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 327 kDa). Approx 300 kDa band observed in lysates of transfected HEK293 and endogenously in lysates of insulinoma cell line B-TC3.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBinds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. May anchor the kinase to cytoskeletal and/or organelle-associated proteins.
Tissue specificityPredominant in many brain structures. Also expressed at medium levels in spleen, thymus, prostate, testis and ovary. Low level expression is seen in heart, kidney, pancreas, skeletal muscle and intestine.
Sequence similaritiesBelongs to the WD repeat neurobeachin family. Contains 1 BEACH domain. Contains 5 WD repeats.
DomainRII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.