Overview

  • Product nameAnti-Neurocan antibody [2Q174]
    See all Neurocan primary antibodies
  • Description
    Mouse monoclonal [2Q174] to Neurocan
  • Tested applicationsSuitable for: ICC, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Full length native core protein (purified) (Rat)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number2Q174
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/5.
IHC-P 1/1000.
WB Use a concentration of 1 - 2 µg/ml.
IP Use a concentration of 1 - 2 µg/ml.

Target

  • FunctionMay modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
  • Tissue specificityBrain.
  • Sequence similaritiesBelongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chondroitin sulfate proteoglycan 3 antibody
    • Cspg3 antibody
    • Ncan antibody
    • NCAN_HUMAN antibody
    • NEUR antibody
    • Neurocan core protein antibody
    • Neurocan proteoglycan antibody
    see all

References for Anti-Neurocan antibody [2Q174] (ab26003)

This product has been referenced in:
  • Haenold R  et al. NF-?B controls axonal regeneration and degeneration through cell-specific balance of RelA and p50 in the adult CNS. J Cell Sci 127:3052-65 (2014). Read more (PubMed: 24860143) »
  • Yao J  et al. Robust cell integration from co-transplantation of biodegradable MMP2-PLGA microspheres with retinal progenitor cells. Biomaterials : (2010). WB ; Mouse . Read more (PubMed: 21030072) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (hippocampus)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment chABC overnight
Specification hippocampus
Blocking step licor fluorescent blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
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Submitted Oct 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"