Overview

  • Product name
    Anti-Neurocan antibody [650.24]
    See all Neurocan primary antibodies
  • Description
    Mouse monoclonal [650.24] to Neurocan
  • Specificity
    The clone number has been updated from (2Q174) to (650.24) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: ICC, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Full length native core protein (purified) (Rat)

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. We would recommend antibody ab125021 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    650.24
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/5.
IHC-P 1/1000.
WB Use a concentration of 1 - 2 µg/ml.
IP Use a concentration of 1 - 2 µg/ml.

Target

  • Function
    May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chondroitin sulfate proteoglycan 3 antibody
    • Cspg3 antibody
    • Ncan antibody
    • NCAN_HUMAN antibody
    • NEUR antibody
    • Neurocan core protein antibody
    • Neurocan proteoglycan antibody
    see all

References

This product has been referenced in:
  • Noguchi A  et al. Juvenile Hippocampal CA2 Region Expresses Aggrecan. Front Neuroanat 11:41 (2017). Read more (PubMed: 28539874) »
  • Haenold R  et al. NF-?B controls axonal regeneration and degeneration through cell-specific balance of RelA and p50 in the adult CNS. J Cell Sci 127:3052-65 (2014). Read more (PubMed: 24860143) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (hippocampus)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
chABC overnight
Specification
hippocampus
Blocking step
licor fluorescent blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
Username

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Verified customer

Submitted Oct 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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