Anti-Neurocan antibody [650.24] (ab31979)

Overview

  • Product nameAnti-Neurocan antibody [650.24]
    See all Neurocan primary antibodies
  • Description
    Mouse monoclonal [650.24] to Neurocan
  • SpecificitySpecific for Neurocan core protein; also reacts with neurocan C fragment
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Embryonic rat brain proteoglycans

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number650.24
  • MyelomaP3x63
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 143 kDa.

Target

  • FunctionMay modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
  • Tissue specificityBrain.
  • Sequence similaritiesBelongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chondroitin sulfate proteoglycan 3 antibody
    • Cspg3 antibody
    • Ncan antibody
    • NCAN_HUMAN antibody
    • NEUR antibody
    • Neurocan core protein antibody
    • Neurocan proteoglycan antibody
    see all

Anti-Neurocan antibody [650.24] images

  • ab31979 staining Neurocan in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab31979 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-mouse polyclonal, used at a 1/300 dilution. Nuclei are counterstained with DAPI.

    See Abreview

References for Anti-Neurocan antibody [650.24] (ab31979)

ab31979 has not yet been referenced specifically in any publications.

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According to the Unigen expression profile of this protein, a good positive control for this protein could be brain or vascular mouse tissue lysates.

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer....

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line)
Specification human glioblastoma cell line
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioblastoma cell line C6)
Specification glioblastoma cell line C6
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioma cell line C6)
Specification glioma cell line C6
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5%
Username

Abcam user community

Verified customer

Submitted Aug 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line D54MG)
Specification human glioblastoma cell line D54MG
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"