The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 130, 270 kDa (predicted molecular weight: 143 kDa).
1/10 - 1/100.
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionMay modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
References for Anti-Neurocan antibody [EPR6397] (ab125021)
This product has been referenced in:
Silver DJ et al. Chondroitin sulfate proteoglycans potently inhibit invasion and serve as a central organizer of the brain tumor microenvironment. J Neurosci33:15603-17 (2013).
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