Overview

  • Product nameAnti-Neurocan antibody [EPR6397]
    See all Neurocan primary antibodies
  • Description
    Rabbit monoclonal [EPR6397] to Neurocan
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Human hippocampus and hypothalamus lysates; Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 3.20 x 10 -11 M Learn more about KD
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR6397
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 130, 270 kDa (predicted molecular weight: 143 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionMay modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
  • Tissue specificityBrain.
  • Sequence similaritiesBelongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chondroitin sulfate proteoglycan 3 antibody
    • Cspg3 antibody
    • Ncan antibody
    • NCAN_HUMAN antibody
    • NEUR antibody
    • Neurocan core protein antibody
    • Neurocan proteoglycan antibody
    see all

Anti-Neurocan antibody [EPR6397] images

  • All lanes : Anti-Neurocan antibody [EPR6397] (ab125021) at 1/10000 dilution

    Lane 1 : Human hippocampus lysate
    Lane 2 : Human hypothalamus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 143 kDa
    Observed band size : 130,270 kDa (why is the actual band size different from the predicted?)
  • ab125021, at 1/250 dilution, staining Neurocan in paraffin-embedded Human brain tissue by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-Neurocan antibody [EPR6397] (ab125021)

This product has been referenced in:
  • Silver DJ  et al. Chondroitin sulfate proteoglycans potently inhibit invasion and serve as a central organizer of the brain tumor microenvironment. J Neurosci 33:15603-17 (2013). Human . Read more (PubMed: 24068827) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"