Overview

  • Product name
    Anti-Neurochondrin antibody
    See all Neurochondrin primary antibodies
  • Description
    Mouse polyclonal to Neurochondrin
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Full length human Neurochondrin protein (NP_055099.1).

  • Positive control
    • Rat brain tissue lysate, Neurochondrin transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab88877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23284858
WB 1/500 - 1/1000. Predicted molecular weight: 79 kDa.

Target

  • Relevance
    Neurochondrin is a leucine rich cytoplasmic protein, which is highly similar to a mouse protein that negatively regulates Ca/calmodulin dependent protein kinase II phosphorylation and may be essential for spatial learning processes.
  • Cellular localization
    Cytoplasm. Cell projection, dendrite. Note: Localizes to somatic regions of neurons.
  • Database links
  • Alternative names
    • AU042419 antibody
    • KIAA0607 antibody
    • M-Sema F-associating protein of 75 kDa antibody
    • MGC93259 antibody
    • MMS10-AE antibody
    • Ms10ae antibody
    • NCDN antibody
    • NCDN-1 antibody
    • NCDN-2 antibody
    • Neurochondrin 1 antibody
    • Norbin antibody
    • OTTHUMP00000065369 antibody
    • OTTHUMP00000065370 antibody
    • OTTHUMP00000065371 antibody
    • Sfap75 antibody
    see all

Images

  • Anti-Neurochondrin antibody (ab88877) at 1/500 dilution + rat brain tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
  • All lanes : Anti-Neurochondrin antibody (ab88877) at 1/500 dilution

    Lane 1 : Neurochondrin transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 79 kDa
    Observed band size : 79 kDa

References

This product has been referenced in:
  • Tang X  et al. Identifying molecular phenotype of nucleus pulposus cells in human intervertebral disc with aging and degeneration. J Orthop Res 34:1316-26 (2016). Read more (PubMed: 27018499) »
  • Tang X  et al. Changes in the molecular phenotype of nucleus pulposus cells with intervertebral disc aging. PLoS One 7:e52020 (2012). ICC/IF ; Rat . Read more (PubMed: 23284858) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Hippocampal neurons)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Hippocampal neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Prachi Ojha

Verified customer

Submitted May 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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