Overview

  • Product nameAnti-NeuroD1 antibody
    See all NeuroD1 primary antibodies
  • Description
    Rabbit polyclonal to NeuroD1
  • Tested applicationsSuitable for: IHC-Fr, IHC-FoFr, ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Apteronotus leptorhynchus
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NeuroD1.

  • Positive control
    • WB: P0 mouse and rat brain tissue lysate. ICC/IF: fixed cultures of murine neurones differentiated for 3 days from murine neocortical precursor cells (E14).

Properties

Applications

Our Abpromise guarantee covers the use of ab16508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20531234
ICC/IF Use a concentration of 1 - 5 µg/ml.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38-43 kDa (predicted molecular weight: 38-43 kDa).

Target

  • FunctionDifferentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
  • Involvement in diseaseDefects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • atonal antibody
    • basic helix loop helix transcription factor antibody
    • BETA 2 antibody
    • Beta cell E box transactivator 2 antibody
    • BETA2 antibody
    • BHF 1 antibody
    • BHF1 antibody
    • bHLHa3 antibody
    • class A basic helix loop helix protein 3 antibody
    • Class A basic helix-loop-helix protein 3 antibody
    • MODY 6 antibody
    • MODY6 antibody
    • NDF1_HUMAN antibody
    • NeuroD antibody
    • NeuroD1 antibody
    • Neurogenic differentiation 1 antibody
    • Neurogenic differentiation factor 1 antibody
    • neurogenic helix loop helix protein NEUROD antibody
    • Neuronal differentiation 1 antibody
    see all

Anti-NeuroD1 antibody images

  • [A] green = ab16508 Rabbit polyclonal antibody to NeuroD1 staining (5ug/ml)) in a fixed culture of murine neurons, differentiated for 3 days, from murine neocortical precursor cells (E14). Neurons shown emanated from the site of a neurosphere (top-left corner) which was removed prior to imaging).

    Alexa Fluor 488 secondary goat anti-rabbit antibody was used for detection (@ 1/400). Strong punctate nuclear and diffuse cytosolic NeuroD1 labelling at is observed.

    [B] red = mouse monoclonal anti-MAP (microtubule marker) with goat anti-mouse Alexa Fluor 568 (1/400) secondary antibody.

    [C] blue = TO-PRO-3 (nuclear) staining

    [D] Overlay of A, B an

  • All lanes : Anti-NeuroD1 antibody (ab16508) at 1 µg

    Lane 1 : P0 Mouse Brain Tissue
    Lane 2 : P0 Rat Brain Tissue
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate (NEGATIVE CONTROL)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38-43 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    NeuroD1 is not expressed in U-87 MG (lane 3 - negative control) 

References for Anti-NeuroD1 antibody (ab16508)

This product has been referenced in:
  • Klisch TJ  et al. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A 108:3288-93 (2011). ICC/IF ; Mouse . Read more (PubMed: 21300888) »
  • Puligilla C  et al. Sox2 induces neuronal formation in the developing mammalian cochlea. J Neurosci 30:714-22 (2010). IHC-P ; Mouse . Read more (PubMed: 20071536) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Human Tissue lysate - whole (Primary pancreatic neuroendocrine tumor PNET)
Specification Primary pancreatic neuroendocrine tumor PNET
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 25 2014

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (primary pancreatic neuroendocrine tumor pNET mouse)
Specification primary pancreatic neuroendocrine tumor pNET mouse
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 25 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (hESC-derived endocrine cells)
Specification hESC-derived endocrine cells
Permeabilization Yes - 0.1 % triton x100
Fixative Paraformaldehyde
Username

Dr. Youngjin Kim

Verified customer

Submitted Mar 24 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 24 2013

Thank you for providing that information.

I have sent you ab60704 as a replacement for ab16508 which did not work as stated on our datasheet. Your new order number is *********.

The new antibody is still covered under our Abpromise ...

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Thank you for your reply.

I would recommend that you try ab60704:

http://www.abcam.com/NeuroD1-antibody-ab60704.html



This antibody has a lot of Abreviews and references associated with it and so you will be able...

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Thank you for completing the follow up survey.

I am sorry that you were unsatisfied with how your complaint was handled; we do try our best to resolve any problems to the best of our ability. As you did not have any success with ab16508, I wo...

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Thank you for contacting Abcam. I am sorry that you are having problems with ab16508. I was wondering if you had tried antigen retrieval techniques when using this antibody. I have looked through the reference you mentioned to find their protocol, whic...

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Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Oct 05 2011

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (hippocampus)
Specification hippocampus
Username

尚三 神野

Verified customer

Submitted Oct 22 2009

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"