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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NeuroD1.
Our Abpromise guarantee covers the use of ab16508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20531234|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 38-43 kDa (predicted molecular weight: 38-43 kDa).|
[A] green = ab16508 Rabbit polyclonal antibody to NeuroD1 staining (5ug/ml)) in a fixed culture of murine neurons, differentiated for 3 days, from murine neocortical precursor cells (E14). Neurons shown emanated from the site of a neurosphere (top-left corner) which was removed prior to imaging). Alexa Fluor 488 secondary goat anti-rabbit antibody was used for detection (@ 1/400). Strong punctate nuclear and diffuse cytosolic NeuroD1 labelling at is observed.
Alexa Fluor 488 secondary goat anti-rabbit antibody was used for detection (@ 1/400). Strong punctate nuclear and diffuse cytosolic NeuroD1 labelling at is observed.
[B] red = mouse monoclonal anti-MAP (microtubule marker) with goat anti-mouse Alexa Fluor 568 (1/400) secondary antibody.
[C] blue = TO-PRO-3 (nuclear) staining
[D] Overlay of A, B an
NeuroD1 is not expressed in U-87 MG (lane 3 - negative control)