Overview

  • Product name
  • Description
    Rabbit polyclonal to NeuroD1
  • Tested applications
    Suitable for: IHC-Fr, IHC-FoFr, ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Apteronotus leptorhynchus
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NeuroD1.

  • Positive control
    • WB: P0 mouse and rat brain tissue lysate. ICC/IF: fixed cultures of murine neurones differentiated for 3 days from murine neocortical precursor cells (E14).

Properties

Applications

Our Abpromise guarantee covers the use of ab16508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20531234
ICC/IF Use a concentration of 1 - 5 µg/ml.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38-43 kDa (predicted molecular weight: 38-43 kDa).

Target

  • Function
    Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
  • Involvement in disease
    Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • atonal antibody
    • basic helix loop helix transcription factor antibody
    • BETA 2 antibody
    • Beta cell E box transactivator 2 antibody
    • BETA2 antibody
    • BHF 1 antibody
    • BHF1 antibody
    • bHLHa3 antibody
    • class A basic helix loop helix protein 3 antibody
    • Class A basic helix-loop-helix protein 3 antibody
    • MODY 6 antibody
    • MODY6 antibody
    • NDF1_HUMAN antibody
    • NeuroD antibody
    • NeuroD1 antibody
    • Neurogenic differentiation 1 antibody
    • Neurogenic differentiation factor 1 antibody
    • neurogenic helix loop helix protein NEUROD antibody
    • Neuronal differentiation 1 antibody
    see all

Anti-NeuroD1 antibody images

  • [A] green = ab16508 Rabbit polyclonal antibody to NeuroD1 staining (5ug/ml)) in a fixed culture of murine neurons, differentiated for 3 days, from murine neocortical precursor cells (E14). Neurons shown emanated from the site of a neurosphere (top-left corner) which was removed prior to imaging).

    Alexa Fluor 488 secondary goat anti-rabbit antibody was used for detection (@ 1/400). Strong punctate nuclear and diffuse cytosolic NeuroD1 labelling at is observed.

    [B] red = mouse monoclonal anti-MAP (microtubule marker) with goat anti-mouse Alexa Fluor 568 (1/400) secondary antibody.

    [C] blue = TO-PRO-3 (nuclear) staining

    [D] Overlay of A, B an

  • All lanes : Anti-NeuroD1 antibody (ab16508) at 1 µg

    Lane 1 : P0 Mouse Brain Tissue
    Lane 2 : P0 Rat Brain Tissue
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate (NEGATIVE CONTROL)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38-43 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    NeuroD1 is not expressed in U-87 MG (lane 3 - negative control) 

References for Anti-NeuroD1 antibody (ab16508)

This product has been referenced in:
  • Ou L  et al. Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo. Mol Med Rep 13:720-30 (2016). Read more (PubMed: 26648540) »
  • Klisch TJ  et al. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A 108:3288-93 (2011). ICC/IF ; Mouse . Read more (PubMed: 21300888) »

See all 7 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Kidney)
Permeabilization
Yes - 0.1% Triron X100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Methanol
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Abcam user community

Verified customer

Submitted Aug 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% Triron X100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Formaldehyde
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Submitted Aug 01 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Aug 01 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Aug 01 2017

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Tissue lysate - whole (Primary pancreatic neuroendocrine tumor PNET)
Specification
Primary pancreatic neuroendocrine tumor PNET
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Jul 25 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (primary pancreatic neuroendocrine tumor pNET mouse)
Specification
primary pancreatic neuroendocrine tumor pNET mouse
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 25 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (hESC-derived endocrine cells)
Specification
hESC-derived endocrine cells
Permeabilization
Yes - 0.1 % triton x100
Fixative
Paraformaldehyde
Username

Dr. Youngjin Kim

Verified customer

Submitted Mar 24 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

Submitted Jan 24 2013

1-10 of 19 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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