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Recombinant fragment with tag: QDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS AEFEKNYAFT , corresponding to amino acids 201-300 of Human NeuroD1
Our Abpromise guarantee covers the use of ab60704 in the following tested applications.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 3 µg/ml.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
|ELISA||Use at an assay dependent concentration. The detection limit for ab60704 is approximately 0.03ng/ml as a capture antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
Immunohistochemical analysis of formaldehyde fixed human cerebellum sections incubated with ab60704 for 20 minutes at 25°C in a concentration of 1/400. The blocking step was performed with 3% H2O2 for 10 minutes at 25ºC. The secondary antibody used was a polyclonal goat anti-mouse/rabbit HRP conjugate, used undiluted.