The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 23269676
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
1/10 - 1/100.
Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
FunctionDifferentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
Involvement in diseaseDefects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Post-translational modificationsPhosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
References for Anti-NeuroD1 antibody [EPR4008] (ab109224)
This product has been referenced in:
Hunter CS & Stein R Characterization of an apparently novel ß-cell line-enriched 80-88 kDa transcriptional activator of the MafA and Pdx1 genes. J Biol Chem288:3795-803 (2013).
Read more (PubMed: 23269676) »
Hunter CS et al. Islet a-, ß-, and d-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor. Diabetes62:875-86 (2013).
Read more (PubMed: 23193182) »