• Product nameAnti-NeuroD1 antibody [EPR4008]
    See all NeuroD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4008] to NeuroD1
  • Tested applicationsSuitable for: EMSA, WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human NeuroD1

  • Positive control
    • Y79 and fetal brain lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


Associated products


Our Abpromise guarantee covers the use of ab109224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration. PubMed: 23269676
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
IP 1/10 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • FunctionDifferentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
    • Involvement in diseaseDefects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • atonal antibody
      • basic helix loop helix transcription factor antibody
      • BETA 2 antibody
      • Beta cell E box transactivator 2 antibody
      • BETA2 antibody
      • BHF 1 antibody
      • BHF1 antibody
      • bHLHa3 antibody
      • class A basic helix loop helix protein 3 antibody
      • Class A basic helix-loop-helix protein 3 antibody
      • MODY 6 antibody
      • MODY6 antibody
      • NDF1_HUMAN antibody
      • NeuroD antibody
      • NeuroD1 antibody
      • Neurogenic differentiation 1 antibody
      • Neurogenic differentiation factor 1 antibody
      • neurogenic helix loop helix protein NEUROD antibody
      • Neuronal differentiation 1 antibody
      see all

    Anti-NeuroD1 antibody [EPR4008] images

    • All lanes : Anti-NeuroD1 antibody [EPR4008] (ab109224) at 1/1000 dilution

      Lane 1 : Y79 lysates
      Lane 2 : Fetal brain lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 40 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)

    References for Anti-NeuroD1 antibody [EPR4008] (ab109224)

    This product has been referenced in:
    • Hunter CS & Stein R Characterization of an apparently novel ß-cell line-enriched 80-88 kDa transcriptional activator of the MafA and Pdx1 genes. J Biol Chem 288:3795-803 (2013). EMSA ; Mouse . Read more (PubMed: 23269676) »
    • Hunter CS  et al. Islet a-, ß-, and d-cell development is controlled by the Ldb1 coregulator, acting primarily with the islet-1 transcription factor. Diabetes 62:875-86 (2013). IP . Read more (PubMed: 23193182) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Non-reduced Denaturing (10% SDS-PAGE)
    Sample Human Cell lysate - whole cell (Lung cancer cells)
    Specification Lung cancer cells
    Blocking step Odyssey Blocking Buffer in TBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C

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    Submitted Oct 03 2014