Anti-NeuroD1 antibody [EPR4008] (ab109224)


  • Product name
    Anti-NeuroD1 antibody [EPR4008]
    See all NeuroD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4008] to NeuroD1
  • Tested applications
    Suitable for: EMSA, WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human NeuroD1

  • Positive control
    • Y79 and fetal brain lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


Associated products


Our Abpromise guarantee covers the use of ab109224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration. PubMed: 23269676
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
    • Involvement in disease
      Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    • Sequence similarities
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • atonal antibody
      • basic helix loop helix transcription factor antibody
      • BETA 2 antibody
      • Beta cell E box transactivator 2 antibody
      • BETA2 antibody
      • BHF 1 antibody
      • BHF1 antibody
      • bHLHa3 antibody
      • class A basic helix loop helix protein 3 antibody
      • Class A basic helix-loop-helix protein 3 antibody
      • MODY 6 antibody
      • MODY6 antibody
      • NDF1_HUMAN antibody
      • NeuroD antibody
      • NeuroD1 antibody
      • Neurogenic differentiation 1 antibody
      • Neurogenic differentiation factor 1 antibody
      • neurogenic helix loop helix protein NEUROD antibody
      • Neuronal differentiation 1 antibody
      see all

    Anti-NeuroD1 antibody [EPR4008] images

    • All lanes : Anti-NeuroD1 antibody [EPR4008] (ab109224) at 1/1000 dilution

      Lane 1 : Y79 lysates
      Lane 2 : Fetal brain lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 40 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)

    References for Anti-NeuroD1 antibody [EPR4008] (ab109224)

    This product has been referenced in:
    • Zhang F  et al. Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells. Neurosci Bull 32:205-16 (2016). Read more (PubMed: 27146165) »
    • Borromeo MD  et al. ASCL1 and NEUROD1 Reveal Heterogeneity in Pulmonary Neuroendocrine Tumors and Regulate Distinct Genetic Programs. Cell Rep 16:1259-72 (2016). WB ; Human . Read more (PubMed: 27452466) »

    See all 4 Publications for this product

    Product Wall

    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Denaturing (10% SDS-PAGE)
    Human Cell lysate - whole cell (Lung cancer cells)
    Lung cancer cells
    Blocking step
    Odyssey Blocking Buffer in TBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C

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    Verified customer

    Submitted Oct 03 2014


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