Overview

  • Product nameAnti-NeuroD2 antibody
    See all NeuroD2 primary antibodies
  • Description
    Rabbit polyclonal to NeuroD2
  • Tested applicationsSuitable for: IHC-Fr, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse NeuroD2.

  • Positive control
    • Brain (Human) Tissue Lysate - adult normal tissue; Cerebellum Mouse Tissue Lysate ; Mouse Hippocampus Tissue Lysate ; P0 Mouse Brain Mouse Tissue Lysate; P7 Mouse Brain Tissue Lysate ; Brain (Human) Tissue Lysate - adult normal tissue; Cerebellum Mouse Tissue Lysate ; Mouse Hippocampus Tissue Lysate ; P0 Mouse Brain Mouse Tissue Lysate; P7 Mouse Brain Tissue Lysate ;

Properties

Applications

Our Abpromise guarantee covers the use of ab104430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/2000. Tissue was perfusion fixed in 10% formaldehyde. Staining appeared more intense without antigen retrieval.
IP Use at an assay dependent concentration.
IHC-P 1/2000 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

Anti-NeuroD2 antibody images

  • All lanes : Anti-NeuroD2 antibody (ab104430) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 5 : P7 Mouse Brain Tissue Lysate
    Lane 6 : Human brain tissue lysate - total protein (ab29466) with Immunising peptide at 1 µg/ml
    Lane 7 : Cerebellum Mouse Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 8 : Mouse Hippocampus Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 9 : P0 Mouse Brain Mouse Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 10 : P7 Mouse Brain Tissue Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 21 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • IHC image of NeuroD2 staining in a formalin fixed, paraffin embedded mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104430, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC-P image of NeuroD2 staining on mouse CA1 hippocampus sections using ab104430 (1:2000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours.

    See Abreview

  • IHC-P image of NeuroD2 staining on rat CA1 hippocampus sections using ab104430 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours

    See Abreview

  • ab104430 staining NeuroD2 in 10% formaldehyde perfusion fixed 6 week old frozen mouse brain section (dentate gyrus). No antigen retrieval was performed. The section was incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104430, 1/2000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

References for Anti-NeuroD2 antibody (ab104430)

This product has been referenced in:
  • Bayam E  et al. Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation. BMC Genomics 16:681 (2015). Mouse . Read more (PubMed: 26341353) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Mouse Cell lysate - whole cell (Neuro2A cell line)
Specification Neuro2A cell line
Treatment Transfected with NeuroD2-myc or empty vector pcDNA4
Total protein in input 100 µg
Username

Prof. Gulayse Ince Dunn

Verified customer

Submitted Jul 11 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"