Overview

  • Product nameAnti-NeuroD2 antibody
    See all NeuroD2 primary antibodies
  • Description
    Rabbit polyclonal to NeuroD2
  • Tested applicationsSuitable for: IHC-Fr, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Gorilla, Chinese Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse NeuroD2.

  • Positive control
    • Brain (Human) Tissue Lysate - adult normal tissue; Cerebellum Mouse Tissue Lysate ; Mouse Hippocampus Tissue Lysate ; P0 Mouse Brain Mouse Tissue Lysate; P7 Mouse Brain Tissue Lysate ; Brain (Human) Tissue Lysate - adult normal tissue; Cerebellum Mouse Tissue Lysate ; Mouse Hippocampus Tissue Lysate ; P0 Mouse Brain Mouse Tissue Lysate; P7 Mouse Brain Tissue Lysate ;

Properties

Applications

Our Abpromise guarantee covers the use of ab104430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/2000. Tissue was perfusion fixed in 10% formaldehyde. Staining appeared more intense without antigen retrieval.
IP Use at an assay dependent concentration.
IHC-P 1/2000 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

Anti-NeuroD2 antibody images

  • All lanes : Anti-NeuroD2 antibody (ab104430) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 5 : P7 Mouse Brain Tissue Lysate
    Lane 6 : Human brain tissue lysate - total protein (ab29466) with Immunising peptide at 1 µg/ml
    Lane 7 : Cerebellum Mouse Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 8 : Mouse Hippocampus Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 9 : P0 Mouse Brain Mouse Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 10 : P7 Mouse Brain Tissue Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 21 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • IHC-P image of NeuroD2 staining on mouse CA1 hippocampus sections using ab104430 (1:2000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours.

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  • IHC-P image of NeuroD2 staining on rat CA1 hippocampus sections using ab104430 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours

    See Abreview

  • ab104430 staining NeuroD2 in 10% formaldehyde perfusion fixed 6 week old frozen mouse brain section (dentate gyrus). No antigen retrieval was performed. The section was incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104430, 1/2000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

References for Anti-NeuroD2 antibody (ab104430)

This product has been referenced in:
  • Bayam E  et al. Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation. BMC Genomics 16:681 (2015). Mouse . Read more (PubMed: 26341353) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Mouse Cell lysate - whole cell (Neuro2A cell line)
Specification Neuro2A cell line
Treatment Transfected with NeuroD2-myc or empty vector pcDNA4
Total protein in input 100 µg
Username

Prof. Gulayse Ince Dunn

Verified customer

Submitted Jul 11 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"