Overview

  • Product nameAnti-NeuroD2 antibody
    See all NeuroD2 primary antibodies
  • Description
    Rabbit polyclonal to NeuroD2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: VKEEGELGGE EEEEEEEEEG LDEAEGERPK KRGPKKRKMT KARLERSKLR, corresponding to N terminal amino acids 73-122 of Human NeuroD2

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:62500.

Target

Anti-NeuroD2 antibody images

  • Anti-NeuroD2 antibody (ab66607) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References for Anti-NeuroD2 antibody (ab66607)

ab66607 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"