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A synthetic peptide corresponding to residues in Human NeuroD2
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Our Abpromise guarantee covers the use of ab109406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|WB||1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).|
|IHC-P||1/250 - 1/500. antigen retrieval is recommended.|
ChIP analysis using ab109406 binding NeuroD2 in cerebral cortex tissue lysate from postnatal day 0 mouse. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with primary antibody (1/200) for 2 hours at 4°C.
Positive control: Known target of NeuroD2, which is the promoter regions of Nhlh2 gene.
Primer sequence for PCR was:
Negative Control: ChIP performed with unrelated antibody (anti-GFP antibody).