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Rabbit polyclonal to Neurofascin
Mouse, RatPredicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 of Human Neurofascin.
- This antibody gave a positive signal in the following tissue lysates:
Rat Brain; Mouse Brain; Mouse Spinal Cord.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 mg/ml. Detects a band of approximately 225 kDa (predicted molecular weight: 138 kDa).
Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Information by UniProt
- KIAA0756 antibody
- Neurofascin antibody
- Neurofascin homolog antibody
Western blot - Anti-Neurofascin antibody (ab90979)
All lanes :
Anti-Neurofascin antibody (ab90979) at 1 µg/mlLane 1 :
Brain (Rat) Tissue Lysate (ab7942
)Lane 2 :
Brain (Mouse) Tissue Lysate (ab27253
)Lane 3 :
Spinal Cord (Mouse) Tissue Lysate (ab50253
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484
) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
138 kDaObserved band size:
225 kDa (why is the actual band size different from the predicted?
)Additional bands at:
32 kDa, 85 kDa. We are unsure as to the identity of these extra bands.Exposure time:
We hypothesize that the observed bands at 225 kDa correspond to the glycosylated form of Neurofascin.
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has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"