Overview

  • Product nameAnti-Neurofascin antibody
    See all Neurofascin primary antibodies
  • Description
    Rabbit polyclonal to Neurofascin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 of Human Neurofascin.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Mouse Spinal Cord.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab90979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 225 kDa (predicted molecular weight: 138 kDa).

Target

Anti-Neurofascin antibody images

  • All lanes : Anti-Neurofascin antibody (ab90979) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate (ab7942)
    Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 3 : Spinal Cord (Mouse) Tissue Lysate (ab50253)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 138 kDa
    Observed band size : 225 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Neurofascin antibody (ab90979)

ab90979 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"